Open access
Jul 2026
Continuous target-specific mutagenesis and rapid gene evolution by diversity-generating retroelements in Escherichia coli
DGRs can be installed in E. coli and reprogrammed for the continuous, iterative mutagenesis of user-defined target genes, and the DGR template RNA can be reprogrammed for gene- and residue-specific mutagenesis, leaving untargeted, adjacent residues unchanged.
Yang Liu, Yang-Qi Gu, Ganesh Agam et al.
· bioRxiv · 0 citations