Engineering robust fermentation hosts remains a barrier to scalable biomanufacturing. In this work we replace canonical mixed-acid fermentation in
Escherichia coli
with a redox‑balanced reverse β‑oxidation (rBOX) pathway that produces medium‑chain fatty alcohols and is bioenergetically equivalent to ethanol ferme...
Bridgie Cawthon, Aidan E. Cowan, Zen Yoshikawa et al.· Nature Communications· 0 citations
Advances in metabolic engineering have enabled the integration of diverse non-native reactions into microbial hosts, providing sustainable alternatives to petrochemical manufacturing. We previously developed a modular polyketide synthase (PKS)-based platform in Streptomyces albus for producing 17 diols, amino alcohols,...
Q. Dan, Xi-Na Wang, Yan Chiu et al.· Metabolic Engineering· 0 citations
Acetate is an attractive renewable two-carbon substrate for microbial biotechnology, but its toxicity limits growth and carbon-use efficiency at process-relevant concentrations. Here, we used adaptive laboratory evolution to improve acetate tolerance in a genome-reduced strain of Pseudomonas putida and combined whole-g...
N. Gurdo, A. Srinivasan, Tommaso Tagliani et al.· bioRxiv· 0 citations
Efficient co-utilization of hexose and pentose sugars from lignocellulose is essential for microbial bioconversion, yet engineered catabolic pathways can be unstable or suboptimal in complex resource environments. Here, we use a Pseudomonas putida strain engineered to catabolize xylose and arabinose to examine how reso...
Sunghwa Woo, H. Lim, B. Norton-Baker et al.· Nature Communications· 0 citations
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