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Author

Xu Li

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#protein folding Oct 2026

Spatially Partitioned Engineering of an Alginate Lyase Overcomes the Stability–Activity Trade-Off via Distal Cap Loop Rigidification and Network Remodeling

The stability–activity trade-off limits engineering of alginate lyases for alginate oligosaccharide production. Here, a spatially partitioned semirational strategy coupled with the greedy accumulated strategy for protein engineering (GRAPE) was applied to an alginate lyase from Vibrio sp. The optimized variant M13 ac...

Meng-Xuan Zhao, Meng-Qi Zhao, Pei-Yi Zhang et al. · 0 citations
Open access Aug 2026

Enhanced heparosan biosynthesis in Corynebacterium glutamicum by systematic metabolic engineering and translation-level fine-tuning

This study systematically engineered Corynebacterium glutamicum, a Generally Recognized as Safe (GRAS) microorganism, to synthesize heparosan via two complementary strategies, which provide valuable references for constructing high-efficiency cell factories for other complex compounds.

Jie Zhang, Ya-Nan Cui, Pei-Yi Zhang et al. · 0 citations

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