Author

Xin-Ming Wang

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Jul 2026

Efficient and precise programmable DNA knock-in without double-strand breaks

Kilobase-scale nickase-targeting (KNIT) editing enables single-nick-based DNA insertion across genomic loci and cell types, supporting insertion of DNA fragments exceeding 10 kb, with high efficiency and minimal unwanted off-target effects.

Yanmin Gao, Yu Ma, Ke-Xin Yu et al. · Nature · 0 citations