Toward point-of-care CRISPR-based diagnostics using split crRNA and split activator/target systems.
Split crRNA and split activator/target strategies have enhanced and advanced CRISPR-based diagnostics (CRISPR/Dx) by requiring a conditional assembly of components. The split crRNA involves dividing the guide RNA (crRNA) itself into multiple inactive fragments that only reassemble to become functional crRNA upon bindin...