Light-responsive materials enabling external modulation of bioactivity and spatial control are highly requested for photopharmacology—a booming research area of modern medicine. We present organo-inorganic hybrids of photoswitchable, bioactive symmetric diamine-phosphine oxides conjugated with luminescent carbon quantum dots (CQDs). The phosphonate compound was found to undergo Z-E isomerization upon 266 nm laser irradiation and exhibit butyrylcholinesterase (BChE) inhibition that increases twofold (15–30%) after photoconversion. Hybrids were fabricated via physical adsorption and chemisorption using different CQD surface groups, and characterized by UV-Vis, luminescence, and FTIR spectroscopy, confirming hybrid formation and retention of functional properties. In both binding modes, the molecules retained photoswitching capability despite steric constraints. All hybrids displayed orthogonal functions: luminescence (excitation at 350 nm) and photomodulation of BChE inhibition (at 266 nm). Remarkably, the binding mode dictated the bioactivity window—chemisorbed hybrids showed narrow 1.5-fold modulation, whereas physisorbed hybrids exhibited ultra-wide >10-fold modulation. This tunable responsiveness, achieved simply by altering the binding mode, demonstrates the exceptional potential of this hybrid design strategy for developing photoswitchable materials with tailored photopharmacological performance.
I. Kolesnikov, Gulia Bikbaeva, A. Egorova et al.· Nanomaterials· 0 citations
The addition of hydroxyapatite (HAP) to electrospun poly-L-lactide (PLLA) scaffolds promotes cell adhesion and differentiation but generally leads to a significant deterioration in mechanical properties due to particle agglomeration. Moreover, the encapsulation of HAP particles within a polymer layer makes them inaccessible to body fluids and cells, thereby limiting the bioactivity of the resulting composite scaffold. In this work, HAP microparticles with median size of 26.3 µm were used to obtain exposed HAP particles on the surface of electrospun PLLA fibers. SEM images and EDX maps revealed that individual particles, particularly the larger ones, were exposed from the polymer scaffold surface. The addition of HAP particles significantly altered the scaffold morphology and structure, increasing the fiber diameter and surface roughness by 2.8–4.1-fold, promoting the formation of fused fiber junctions, and inducing the appearance of semicrystalline PLLA domains. These structural changes significantly improved the mechanical properties of the scaffolds. Specifically, the tensile strength and Young’s modulus of the prepared scaffolds are increased by 2.3–3.8-fold following HAP incorporation. Compared with neat PLLA scaffolds, HAP-containing scaffolds exhibited 1.2–1.4-fold higher osteocalcin and osteopontin expression by human adipose-derived mesenchymal stromal cells (hADSCs). Compared to tissue culture plastic, the expressions of osteocalcin and osteopontin on the composite scaffolds were 7.1–7.9-fold and 2.8–3.0-fold higher, respectively.
A. Badaraev, M. Buldakov, Vladislav R. Bukal et al.· Polymers· 0 citations
The NCRPF nanotherapeutic system provides a powerful new paradigm with high translational potential for the complete eradication of breast cancer, and both in vitro and in vivo results confirm that this combined strategy achieves complete tumor eradication with favorable biosafety.
