Two-photon light-sheet microscopy with isotropic near-diffraction-limited resolution in three dimensions
Summary Achieving isotropic high-resolution three-dimensional imaging across a large field of view (FOV) remains a key challenge in two-photon light-sheet microscopy (TPLSM). To address this limitation, we developed a TPLSM system that stitches multiple short Bessel beams into a large, ultrathin illumination light shee...