SEEKER: A genome-scale library-on-library screening platform for deciphering T cell recognition of antigen
Decoding which antigens activate a given T cell receptor (TCR) is a central challenge in immunology. We present SEEKER, a functional genome-scale library-on-library screening platform built on three elements: a Jurkat-derived T-APC cell (TAPCell) that combinatorially expresses one TCR and one peptide–MHC drawn from sep...