Owing to their excellent biocompatibility and diverse bioactivities, plant-derived polysaccharides have garnered increasing attention. As key dietary components, plant-derived polysaccharides have shown potential as nutritional interventions for supporting gastrointestinal health in various experimental models. This review systematically presents the latest insights into the multifaceted mechanisms of plant-derived polysaccharides over the past 5 years. Notably, inconsistencies among various experimental models regarding their effects on enhancing intestinal mechanical barrier function through the upregulation of tight junction proteins, modulation of host immune responses via cytokine regulation, and their role as prebiotics in improving beneficial gut microbiota and producing short-chain fatty acids are highlighted. This paper critically elucidates how monosaccharide composition, molecular weight, glycosidic bonds, and chemical modifications determine biological activity. Nonetheless, significant translational challenges persist: discrepancies exist between effective doses observed in animal models and their tolerability in humans, largely because of the lack of standardized protocols. A critical evaluation of these methodological differences and their implications for translational applications is necessary. Furthermore, we propose an integrated ‘crop-to-clinical’ perspective that links agricultural sourcing, processing stability, and clinical validation. Finally, this review emphasizes the necessity of rigorous quality assurance systems and analyses the complex global regulatory frameworks governing functional food applications.
Ting Ju, Yishu Yin, Jiayu Wang et al.· Quality Assurance and Safety...· 0 citations
Dairy peptides and plant polyphenols attract significant attention for their bioactive properties; however, the synergistic antioxidant mechanism of buttermilk peptides and myricetin remains unclear. In this study, buttermilk proteins from Lacprodan MFGM-10, a milk fat globule membrane (MFGM)-enriched buttermilk powder, were subjected to multi-enzyme hydrolysis using papain (Pap), alkaline protease (AP), and neutral protease (NP) at an enzyme concentration of 6000 U/g in different combinations. The results showed that the synergistic application of enzymes significantly increased the degree of hydrolysis (DH), with the AP group reaching a peak DH of approximately 42% at 150 min. Two candidate peptides, WGSPP and SWPWQ, were selected from the screened buttermilk peptide fractions. Through Caco-2 transmembrane transport validation and molecular docking, it is confirmed that these peptides can non-competitively bind to acetylcholinesterase (AChE) via molecular docking, with binding energies of -12.7 and -11.1 kcal/mol, respectively. Additionally, myricetin exhibits a strong binding energy, with a binding energy of -9.5 kcal/mol. In an L6 myoblast injury model induced by rotenone, combined treatment significantly enhances AChE inhibitory rate, reduces reactive oxygen species (ROS) and malondialdehyde (MDA) levels, restores superoxide dismutase (SOD) activity and the NAD+/NADH ratio, and improves mitochondrial membrane potential and ATP levels, without demonstrating cytotoxicity. Buttermilk-derived peptides and myricetin exert synergistic antioxidant effects by improving redox homeostasis and mitochondrial function, thereby alleviating oxidative stress-induced injury in rotenone-induced L6 cells. These findings provide preliminary evidence for the potential role of these compounds in oxidative stress-associated skeletal muscle health; however, direct sarcopenia-related endpoints were not evaluated in the present study.
Wenjing Niu, Xiao-Lin Liu, Jiexia Bai et al.· Journal of Food Science· 0 citations
We use cookies to run the site and, with your consent, for analytics and to show ads.
See our Cookie Policy.
