Flaxseed (Linum usitatissimum L.) is widely recognized as a functional food due to its rich nutritional composition; however, the metabolic basis underlying seed coat color variation remains unclear. In this study, we performed a broad-targeted metabolomics analysis of six flaxseed varieties (three brown and three yellow) grown at two distinct geographical locations. A total of 1456 metabolites were identified, among which flavonoids and phenolic acids were the most abundant differentially accumulated compounds. Eight metabolites consistently exhibited the largest differences across all comparison groups, and thus warrant further investigation as candidate markers in genetically controlled populations. Correlation analysis further indicated that these metabolites are associated with seed coat pigmentation and other developmental processes. Pathway enrichment revealed that the biosynthesis of caffeic acid derivatives, ferulic acid derivatives, and flavones/flavonols was differentially regulated between yellow and brown seeds. Collectively, our findings provide a comprehensive metabolomic landscape of colored flaxseeds and provide a foundation for future studies aimed at breeding flaxseed varieties with targeted color and nutritional traits.
Wen-Juan Li, Jing Sun, Yang Yang et al.· Foods· 0 citations
Salt stress is a major constraint affecting crop productivity, and understanding plant metabolic responses is essential for improving tolerance. In this study, we examined the metabolic pathways of flavonoids in flax ( Linum usita-tissimum L.) roots using a salt-tolerant genotype (R40) and a salt-sensitive genotype (R24). Plants were subjected to 150 mmol L⁻¹ NaCl, and changes in metabolites were analyzed using a widely targeted metabolomics approach. A total of 732 metabolites were detected, including 332 that increased and 400 that decreased under salt stress. Among these, 163 flavonoids were further analyzed to explore their role in stress response. KEGG pathway analysis revealed significant changes in flavonoid-related pathways, with 9 pathways upregulated, 10 down-regulated, and 4 showing mixed responses. Key metabolites with strong antioxidant properties, such as quercetin and epicat-echin, were identified as important components of the plant’s defense mechanism. Notably, clear differences were observed between the tolerant and sensitive genotypes in terms of flavonoid accumulation and metabolic patterns. These findings highlight the important role of flavonoids in helping flax cope with salinity stress and provide useful insights for developing salt-tolerant varieties.
Minglu Yuan, Baoxing Wan, Wei Zhao et al.· Journal of Ecological Engine...· 0 citations
We use cookies to run the site and, with your consent, for analytics and to show ads.
See our Cookie Policy.
