Synthesis-driven reverse metabolomics reveals 3-hydroxy N-acyl amides as gut microbial molecules
ABSTRACT 3-Hydroxy N-acyl amides are bioactive lipids with reported anti-obesity and glucose-regulating effects, yet they are rarely detected in untargeted metabolomics studies because they are largely absent from existing spectral reference libraries. To address this gap, we synthesized an MS/MS spectral resource comp...