Veronika Raindlová

Site-Specific Enzymatic Syntheses of DNA Bearing Several Modifications From RNA or DNA Templates.

Two approaches for the enzymatic synthesis of DNA oligonucleotides (ONs) bearing multiple site-specific modifications were explored. The first approach based on repeated single nucleotide extension (SNI) and reverse transcription from RNA template with Bst 3.0 DNA polymerase followed by digestion and exchange of templa...