Veit Hornung

SPARCS enables scalable recovery of complex image-based phenotypes for genetic screening.

Forward genetic screening links genotype to phenotype by introducing random genetic perturbations and identifying phenotype-altering mutations. Although genome-scale screens are routine for simple phenotypes in cultured cells, extending them to complex image-based phenotypes remains challenging. Here, we present spatia...

Mitochondrial protein import couples proteostasis failure to mitochondrial permeabilization

It is established that mitochondrial protein import couples translation-associated proteotoxic stress to mitochondrial injury and regulation of import flux as a determinant of mitochondrial integrity during proteostasis failure.

Optimizing CRISPR/Cas9 genome editing in primary human hematopoietic cells to advance studies into HIV biology