Author

Vangelis Daskalakis

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Open access Sep 2026

A dual gating mechanism controls target‐strand cleavage in Cas12j: Implications for engineering efficient nickases

These findings provide a mechanistic basis for tuning Cas12j activity along the nuclease‐to‐nickase spectrum, supporting the rational engineering of genome‐editing tools with controlled target‐strand cleavage kinetics, and offering a path toward applications that bypass dependence on Non‐Homologous End Joining (NHEJ or...

Ilias G. Karvounis, Vangelis Daskalakis · Protein Science · 0 citations

Review Open access Aug 2026

A practical guide to investigating biomolecular condensates: a comment from the plant community.

This review aims to establish a foundation for rigorous, reproducible, and conceptually coherent research in condensate biology of plants and beyond, with emerging implications for crop genetic improvement and synthetic biology applications.

Jiaxuan Peng, J. Agudo-Canalejo, Monika Chodasiewicz et al. · Science China Life Sciences · 0 citations