V. Gladyshev

Transient transcriptomic rejuvenation during intestinal and liver regeneration in mice

Aging vs. biological age: Conceptual considerations for age reversal claims.

Whether reversal of biological age and/or aging is possible is among the most actively debated topics in the field of aging. Here, we consider the meaning of biological age reversal and its burden of proof, focusing on foundational issues and the language we use to debate these questions.

Longitudinal alignments and syntheses of multimodal clinical data for personalized medicine with the PULSE framework

Multimodal models capable of imputing diverse data used in single-cell biology studies provide potential foundational opportunities in clinical practice. However, patient data uniquely comprise longitudinal mosaic measurements that reflect underlying physiological dynamics and exhibit temporal covariation, demanding a...

Transplanted hearts assimilate the recipient’s biological age

Background Despite the growing demand for transplantation, a shortage of donor organs persists, limiting access to this lifesaving procedure. To meet the increasing demand, organs are frequently transplanted across an age discrepancy between donor and recipients. Methods We delineated biological age dynamics in heteroc...

Integration of proteomic aging clocks in a phase 2a clinical trial supports simultaneous geroprotective assessment.

This work supports the goal of dual-purpose clinical trial designs that integrate aging endpoints into studies for specific disease indications by identifying potential anti-aging shifts in senescence and metabolic processes alongside the anti-fibrotic activity of rentosertib.

MA-5 attenuates disease-associated transcriptomic aging and pathological microglial states in Gaucher disease

Overall, MA-5 suppresses neuroinflammation and reverses accelerated transcriptomic aging, supporting its potential as a therapeutic strategy for GD and other lysosomal storage disorders, as well as for diseases characterized by mitochondrial dysfunction, chronic inflammation, and pathological accumulation.

Measuring the Effects of Pharmacological Compounds on Mitochondrial Morphology in Mouse Aging Models.

Methods for performing subcutaneous osmotic pump implantations in aged mice for the stable delivery of hydrophobic pharmacological compounds and an automated pipeline for the three-dimensional segmentation, classification, and quantitative measurement of mitochondrial structures may be useful for quantitatively assessi...