Open access
Aug 2026
Two activation heat capacity regimes underlie temperature-dependent catalysis in homologous archaeal ADP-dependent kinases
This work characterized the glucokinase activity of three homologous bifunctional ADP-dependent PFK/GK enzymes and presented a two-pathway model incorporating a conformational equilibrium in which free enzyme and enzyme-substrate complex populate two catalytically competent conformations.
Ignacio Aravena-Valenzuela, P. Maturana, Leslie Hernández-Cabello et al.
· bioRxiv · 0 citations