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Sponsored Artificial Intelligence: A Modern Approach, Global Edition Artificial Intelligence: A Modern Approach, Global Edition

Author

Tongbo Wu

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Jul 2026

High-specificity gene point mutation detection by PAM-free Cas12a system with double-stranded substrate positioning-unwinding.

A PAM-free Cas12a system with double-stranded substrate positioning-unwinding (dsPU-Cas12a), wherein "bubble" structures formed by unpaired base pairs release partial single-stranded target strand as a toehold, providing a high-performance tool for clinical gene point mutation detection.

Zhujun Liu, Jinjin Wang, Zhengguang Yang et al. · 0 citations

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