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Author

Tong Yan

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Open access Oct 2026

One-Pot Encapsulation of a Robust α-L-Rhamnosidase Mutant in Cu-MOF: A Recyclable Designed Biocatalyst for High-Yield Conversion of Icariin to Icariside I

Icariside I is a pharmacologically important prenylated flavonoid glycoside, yet its efficient biocatalytic production from icariin is hindered by the limited stability and recyclability of the enzymes involved. In this study, an α-L-rhamnosidase mutant R783A (Rha R783A) was heterologously expressed, purified, and immo...

Hao-Yu Jia, Tong Yan, Lu-Ran Wang et al. · 0 citations
Jul 2026

Site-directed mutagenesis of α-L-rhamnosidase boosts its deglycosylation activity for the production of bioactive flavonoid glycosides.

The rational engineering of an α-L-rhamnosidase (DthRha) to address limitations and enhance its performance for flavonoid production highlights the R783A mutant as a robust and thermally stable biocatalyst with great potential for the sustainable production of bioactive flavonoids.

Haoyu Jia, Luran Wang, Tong Yan et al. · 1 citation

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