Author

Tom Desmet

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Open access Sep 2026

Cellulase-secreting yeast can substantially reduce commercial enzyme requirements in lignocellulosic ethanol production

Cellulase enzyme secretion by cells of 2G S. cerevisiae yeast is considered a promising strategy to reduce the prohibitive cost of commercial cellulase cocktails in lignocellulosic bioethanol production. Here, we have developed Cellusec ® strains by engineering enzyme secretion capacity into an industrial l...

Bart Thevelein, M. Demeke, Tom Desmet et al. · Biotechnology for Biofuels a... · 0 citations

Open access Jul 2026

Discovery of a multifunctional chitinase-cellulase from Thermococcus chitonophagus with expanded polysaccharide specificity.

TcChi is a thermostable, multifunctional biocatalyst capable of degrading chitin, chitosan, cellulose, and β-1,3-glucan from a single protein scaffold, making it a promising candidate for consolidated biomass deconstruction and waste valorization.

Alex Windels, S. Dhaene, Tom Desmet · Biotechnology for Biofuels a... · 0 citations

Jul 2026

Efficient high-temperature simultaneous saccharification and fermentation of lignocellulosic substrates enabled by engineering thermotolerance in an enzyme-secreting industrial Saccharomyces cerevisiae strain.

The high cost of commercial enzyme cocktails remains a major barrier for lignocellulosic (second-generation) bioethanol production. Simultaneous saccharification and fermentation (SSF) at elevated temperatures using enzyme-secreting yeast can reduce enzyme demand, but is constrained by the limited thermotolerance of in...

Bart Thevelein, M. Demeke, S. De Graeve et al. · Bioresource Technology · 0 citations