Open access
Jul 2026
A hybrid system enables plasmid copy number control in yeast
A programmable PCN platform for S. cerevisiae based on the endogenous 2μ plasmid achieves up to 20 copies per cell with enhanced homogeneity and stability and establishes a well quantified PCN regulation toolkit for yeast, addressing instability from multiple copies and enabling gene dosage control across DNA, RNA, and protein levels.
Anni Li, Q. Zhao, Zhu Yang et al.
· Nature Communications · 0 citations