ABSTRACT Enterococcus faecalis is a gram-positive commensal bacterium of the human gut microbiome and an opportunistic pathogen responsible for many hospital-acquired infections. Despite the clinical importance of E. faecalis, how gene and protein expression are coordinated with growth remains poorly defined. Here, we...
M. M. Mitchener, Caleb M. Anderson, Mark Veleba et al.· mBio· 0 citations
In eubacteria, decoding of isoleucine codon AUA requires a specialized tRNA (tRNAIle2) modified with lysidine (k2C) at the anticodon wobble position (C34), which switches decoding specificity from methionine (AUG) to isoleucine (AUA). Recently, aminovaleramide cytidine (ava2C) was discovered at the same tRNA position i...
Jing-Jing Sun, Jun-Zhou Wu, Yi-Feng Yuan et al.· Nature Chemical Biology· 0 citations
Oncogenes and tumor-suppressor genes play opposing roles in cancer biology to promote and restrict growth, respectively. Codon usage patterns interface with tRNA modifications to control translation, leading to gene-specific codon signatures with regulatory potential. As such, codon-biased translational regulation has...
Chetna Mathur, Evan T. Davis, Dylan J. Ehrbar et al.· bioRxiv· 0 citations
Escherichia coli uses wobble uridine (U34) modifications to tune codon decoding, but how individual tRNA writer enzymes shape gene expression remains unclear. Here, we identify MnmA, the U34 thiolation enzyme for tRNALys, tRNAGln, and tRNAGlu, as a central regulator linking codon-directed translation to regulon control...
H. Omeoga, Dylan J. Ehrbar, Chetna Mathur et al.· bioRxiv· 0 citations
ABSTRACT Enterococcus faecalis is a gram-positive commensal bacterium of the human gut microbiome and an opportunistic pathogen responsible for many hospital-acquired infections. Despite the clinical importance of E. faecalis, how gene and protein expression are coordinated with growth remains poorly defined. Here, we...
M. M. Mitchener, Caleb M. Anderson, Mark Veleba et al.· mBio· 0 citations
Transfer RNA (tRNA) modifications regulate codon-biased translation and enable rapid translational adaptation to environmental stresses in diverse organisms. While these mechanisms have been extensively characterized in Saccharomyces cerevisiae, little is known about the epitranscriptomic landscape of nonconventional...
Alexander J. B. Reading, Chi-Kong Chan, R. Raman et al.· Chemical Research in Toxicol...· 0 citations
This work develops a potential method to construct more biostable DNA nanostructures and 3D crystals in a simple one-tube process by assembly of a wide variety of DNA nanostructures and 3D crystals in a hydrated ionic liquid instead of magnesium at constant moderate temperatures.
It is demonstrated that maximizing recombinant protein production requires optimization of both yield and structural quality and establish complementary strategies for improving secreted protein expression in Pichia.
