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Thierry Joet

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Open access Aug 2026

Development of efficient methods for protoplast-to-plant regeneration in Coffea arabica and successful CRISPR-Cas9 editing in protoplasts

An efficient protocol for protoplast isolation from embryogenic calli and protoplast-to-plant regeneration in the allotetraploid species Coffea arabica is presented and polyethylene glycol-mediated transfection of coffee protoplasts with a CRISPR–Cas9 plasmid targeting the coffee xanthosine methyltransferase gene involved in caffeine biosynthesis is demonstrated.

Lucas Laflaquiere, H. Etienne, Thierry Joet et al. · 0 citations

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