Two Glu/Asp Residues Cooperatively Mediate an Early Step of ATP Hydrolysis in GHKL ATPases MutL and GyrB
The catalytic mechanism of GHKL ATPases is refined and variants of uncertain significance in these DNA mismatch repair proteins substantially reduced the ATPase activity, indicating functional impairment, and a structural and functional framework for interpreting disease-associated variants in GHKL ATPases is provided.
Kenji Fukui, Ayaka Shibuya, T. Murakawa et al.
· bioRxiv · 0 citations