Helicolysins emerge as a new family of metzincin metallopeptidases in host-pathogen interactions.
The Helicobacter pylori Hom family of outer-membrane proteins (OMPs) has been implicated in virulence-related processes. Here, we present a structural and functional characterization of HomA from H. pylori strain 26695. Comparative in vivo analyses of the wild-type strain and a homA deletion mutant demonstrate a role for HomA in focal adhesion kinase phosphorylation at Y397 and in bacterial adhesion to human leukaemia-derived MEC-1 cells. Recombinant production of several HomA variants identified a soluble ectodomain (ECDHomA) that harbours the conserved zinc-binding motif of metzincin metallopeptidases (MPs) and displays dimerization capacity, acid resistance, and immunomodulatory activity by triggering a pro-inflammatory response in Raji human B-lymphoblastoid cancer cells. Site-directed mutagenesis of the zinc-binding motif confirmed its contribution to zinc coordination and thermal stability. Structural analysis of ECDHomA revealed a two-domain organization comprising an N-terminal accessory β-sandwich domain (ND) and a C-terminal catalytic domain (CD), in which a non-canonical 'Thr-turn' replaces the hallmark metzincin 'Met-turn'. Integrating bioinformatics with AI-based structure predictions uncovered putative HomA homologues across 44 proteomes, predominantly from Gram-negative bacteria. Structural and biochemical characterization of the closest non-Helicobacter homologue from the oral pathogen Capnocytophaga canimorsus, dubbed canilysin, revealed an architecture equivalent to ECDHomA and highly specific MP activity. Together, these findings define the Hom family and canilysin as helicolysins, a previously uncharacterized metzincin subfamily distinguished by a conserved Thr-turn and an accessory ND, and implicates these proteins in host-pathogen interactions, adhesion, and immunomodulation.