Efficient Endogenous Tagging in the Sea Urchin, Lytechinus pictus, Using CRISPR/Cas9-mediated Split-Fluorescent Protein Knock-In
A transgenic Lytechinus pictus line that constitutively and ubiquitously expresses a large fragment of mNeonGreen is used, providing a streamlined, scalable method for endogenous protein visualization in echinoderm embryos and a valuable resource for studying gene function, morphogenesis, and toxicant response in this classic developmental model.