Reconciling Cryo-EM and Crystallographic Views of RNA Folding through Atomistic Structure-Based Simulations: Roles of Ionic Conditions and Truncation of Flexible Elements.
Discrepancies between biomolecular structures resolved by cryo-electron microscopy (cryo-EM) and X-ray crystallography (XRD) arise from differences in ionic conditions and construct design, yet how these shape RNA folding remains unresolved. The SARS-CoV-2 frameshifting stimulatory element provides a representative cas...