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Author

Stevanie Stevanie

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Open access Jul 2026

Genomically integrated orthogonal translation system in Escherichia coli enables production of functional modified [NiFe]-hydrogenases

The present work lays the groundwork for the bio-orthogonal engineering of hydrogenases and related hybrid biocatalysts by employing CRISPR/Cas9-mediated genome editing to integrate a psychrophilic pyrrolysyl-tRNA synthetase/tRNA pair into the Escherichia coli BL21 genome.

Qin Fan, Stevanie Stevanie, S. Frielingsdorf et al. · 0 citations

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