Combining Ability Analysis for Seed Yield and Associated Traits in Indian Mustard (Brassica juncea L. Czern & Coss.)
Indian mustard belongs to the family Brassicaceae and the genus Brassica and is a major oilseed crop cultivated in India. Worldwide, it is the third-ranked oilseed crop in terms of consumption after palm oil and soybean. However, productivity remains below its potential because of various biotic and abiotic stressors coupled with a narrow genetic base, highlighting the need to broaden the genetic base and exploit heterosis to develop superior cultivars. Therefore, in the present study, general and specific combining ability analyses were conducted to assess the combining abilities of the parents and the performance of hybrids at the Zonal Agricultural Research Station, Morena, Rajmata Vijayaraje Scindia Agricultural University, Gwalior, M. P., India, during Rabi 2026. During the present investigation, 16 yield-attributing traits were evaluated using 10 lines, 3 testers, and their thirty F1 hybrids developed through a line × tester mating design to estimate the magnitude and direction of combining abilities. Among the lines and testers, GCA effects showed that parents viz., Pusa Bold, Rohini, Varuna, and Vardhan were good general combiners because they exhibited desirable negative and highly significant GCA effects for days to 50% flowering, days to siliquae initiation, and plant height. However, Pusa Bold was recognized as a good general combiner because it displayed positive and highly significant GCA effects for the maximum number of quantitative traits, including seed yield per plant and harvest index. The cross-combination Pusa Jagannath × PAB-2014-4 had the highest SCA effect for seed yield per plant, biological yield, number of siliquae per plant and number of primary branches per plant, while Maya × PAB-2014-8 showed high SCA effects for seed yield per plant, biological yield and number of siliquae on the main raceme.