Aug 2026
A Dual-Dimensional CRISPR Toolkit Enables One-Step High-Efficiency Multiplex Genome Editing in Komagataella phaffii.
A CRISPR toolkit - Efficient Multi-Gene Editing System 3.0 (EMGES 3.0) - which enabled one-step large-fragment integration coupled with multiplex gene knockout coupled with multiplex gene knockout is established and provides a robust technical foundation for complex compounds biosynthesis and high-yield industrial stra...
Zhixian Yu, Kaidi Chen, Gulikezi Maimaitirexiati et al.
· Metabolic Engineering · 0 citations