An Integrated Workflow for Improving Thermostability: Mechanism and Engineering of GH11 Xylanases via Ser/Thr Substitutions.
Thermostability is critical yet challenging to engineer in industrial enzymes. Here, we present an integrated workflow for enhancing the thermostability of GH11 xylanases through systematic Ser/Thr optimization. Gibbs free energy calculations on XynCDBFV identified 169 stabilizing single-site mutations involving Ser/Th...