Proteome stability and dynamic protein complex profiling identified EEF1A1 as a novel translation regulator of autophagy and mitophagy
This work integrates thermal proteome profiling with co-aggregation analysis to monitor protein thermal stability and complex assembly dynamics in response to two canonical inducers: Torin 1, which activates autophagy via mTOR inhibition, and CCCP, a mitochondrial uncoupler that triggers mitophagy.