Shu-Wen Chen

An activator-free method for the synthesis of nucleotide 5′–5′ triphosphate mRNA capping agent

The traditional synthesis of nucleotide 5′–5′ triphosphate linkages involves a “2 + 1” phosphate coupling mediated by a large excess of metal salts, where the salt removal is a labor and waste-intensive step. We have omitted the metal salts from this reaction by modulating the solubility and reactivity of the monophosp...