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It is demonstrated that NEK2 is a critical regulator of porcine oocyte meiotic maturation through its roles in SAC regulation, K-MT attachment, cytoskeletal dynamics, and the DNA damage response.
A dual-mode genome regulation platform using ISDge10 TnpB effectors and engineered ωRNAs is established, in which modulation of the ωRNA guide length enables switching between programmable transcriptional activation and genome editing.
Efficient delivery of CRISPR components remains a major determinant of genome editing outcomes. In this study, we compared conventional lipofection with magnetic nanoparticle-assisted gene delivery (magnetofection) for CRISPR-mediated genome editing efficiency using SpCas9 and AsCas12a systems. Based on the average val...
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