Three-dimensional ultrastructural analysis of fertilization-defective Arabidopsis mutants using STEM and array tomography.
Double fertilization in angiosperms is completed by the fusion of two sperm cells with the egg and central cells. While sperm membrane proteins such as GEX2, DMP8/9, and GCS1/HAP2 have been identified as key regulators of gamete attachment and fusion, their specific ultrastructural roles within the embryo sac have rema...