Scott Harper

Effect of nucleic acid extraction methodology and PCR chemistry on the detection of 'Candidatus Phytoplasma pruni' in sweet cherry.

Early and accurate detection of 'Candidatus Phytoplasma pruni' is essential for the control of X-disease in Prunus species, but this is made difficult by pathogen biology and the ability to consistently recover nucleic acids, both of which increase the likelihood of false negatives. Here we examined the latter, using h...