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Sarah J. Harmych

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Review Open access Sep 2026

Beyond the Monolayer: Leveraging 3D Culture Systems to Decode Mechanisms of Drug Resistance and Therapeutic Vulnerabilities in Colorectal Cancer

ABSTRACT Two‐dimensional (2D) monolayer cultures remain the workhorse of colorectal cancer (CRC) research and high‐throughput screening (HTS), yet they poorly recapitulate tissue architecture, extracellular matrix (ECM) cues, metabolic gradients, and multicellular heterogeneity that govern drug response in vivo. Over t...

Sarah J. Harmych, Bhuminder Singh · 0 citations

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