S. J. Singha Roy

Repurposing engineered aminoacyl-tRNA synthetases for proximity-dependent protein labeling through the release of aminoacyl-AMP intermediate

Over the past two decades, engineering efforts have yielded aminoacyl-tRNA synthetases (aaRSs) capable of charging diverse noncanonical amino acids (ncAAs). Here, we report an unexpected and exploitable activity of these engineered aaRSs: In the absence of their cognate tRNA, aaRSs can release the ncAA-AMP intermediate...