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Author

Rui-Jie Deng

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#protein folding Oct 2026

High-Gain Bacterial RNA Imaging via High-Density Protein Fluorescence In Situ Hybridization

Fluorescence in situ hybridization (FISH) is important for bacterial genotypic analysis; however, the limited number of labels per probe results in insufficient signal gain, compromising the detection of low-abundance RNAs. Here, we present high-density protein FISH (HD-FISH) for high-gain bacterial RNA imaging. Inst...

Qiu-Yu Luo, Jie-Mei Zhuang, Sha Deng et al. · 0 citations
Review Sep 2026

Advances in cascaded CRISPR for preamplification-free nucleic acid assays.

The design principles and working mechanisms of cascaded CRISPR strategies, encompassing Enzyme-Coupled cascades, Multi-effector class 2 CRISPR cascades, and Type III CRISPR-mediated cascades are introduced, including integration with digital detection platforms and AI-assistant algorithms are discussed.

Ruo-Nan He, Lu Miao, Rui-Jie Deng et al. · 0 citations
Review Aug 2026

Engineering CRISPR for Point-of-Care Tests.

This work systematically review the recent advancements in CRISPR engineering strategies aimed at improving detection sensitivity and specificity, eliminating the dependence on preamplification, and enabling robust POC deployment.

Yu-Xiu Ma, Yi Deng, Jia-Ying Xu et al. · 0 citations

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