Fluorescence in situ hybridization (FISH) is important for bacterial genotypic analysis; however, the limited number of labels per probe results in insufficient signal gain, compromising the detection of low-abundance RNAs. Here, we present high-density protein FISH (HD-FISH) for high-gain bacterial RNA imaging. Inst...
Qiu-Yu Luo, Jie-Mei Zhuang, Sha Deng et al.· Analytical Chemistry· 0 citations
The design principles and working mechanisms of cascaded CRISPR strategies, encompassing Enzyme-Coupled cascades, Multi-effector class 2 CRISPR cascades, and Type III CRISPR-mediated cascades are introduced, including integration with digital detection platforms and AI-assistant algorithms are discussed.
Ruo-Nan He, Lu Miao, Rui-Jie Deng et al.· Biosensors & bioelectronics· 0 citations
This work systematically review the recent advancements in CRISPR engineering strategies aimed at improving detection sensitivity and specificity, eliminating the dependence on preamplification, and enabling robust POC deployment.
Yu-Xiu Ma, Yi Deng, Jia-Ying Xu et al.· ACS Sensors· 0 citations
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