Lipid nanoparticles (LNPs) are a leading platform for mRNA delivery, yet improving their performance often requires complex chemical modification or reformulation of core components. Here, we report a modular peptide-coating strategy for post-formulation engineering of LNP interfaces through physical assembly. A modu...
Chuan-Mei Tang, Yu-Zhi Ye, Ji-Wei Min et al.· Biomacromolecules· 0 citations
Efficient and safe delivery of nucleic acids remains a major challenge for clinical translation of gene therapies. Here, we report de novo-designed α-helical peptides that coassemble with lipids and nucleic acids to form core-shell, virus-like nanoparticles (VLNs). The peptides combine a cationic N-terminus for nucleic...
Mingshan Han, Jintao Feng, Xi Rong et al.· Langmuir· 0 citations
A directed engineering strategy is employed combining energy-based screening and protein language models to obtain a triple mutant, TIG, which enhances residue flexibility and remodels the ATP-binding pocket, thereby improving DNA-binding pathway connectivity and strengthening the coupling between ATP hydrolysis and DN...
Ling-Song Li, Lin Zhang, Dong-Xia Li et al.· International Journal of Bio...· 0 citations
We report the rational engineering of a two-α-helix protein via site-directed mutagenesis, wherein histidine residues at the native catalytic active site were substituted with cysteines and tyrosine residues were introduced in close proximity to these cysteine residues. This modification enabled the site-directed red...
Yaoyu Liang, Jingsong Ma, Lei Shang et al.· ACS Applied Nano Materials· 0 citations
A multifunctional peptide-based antibacterial hydrogel (PAHG) for treating infected wounds, constructed through the incorporation of Cys-Arg-NH2 (CR)-Ag nanoassemblies into a bioactive matrix with excellent antibacterial activity against Escherichia coli and methicillin-resistant Staphylococcus aureus.
Heng Ge, Wen Yuan, Yulin Sun et al.· Journal of materials chemist...· 0 citations
The accumulation of poly(ethylene terephthalate) (PET) waste in the environment poses a severe ecological threat. While extensive research has focused on high-performance PET degradation by thermophilic enzymes, PET hydrolases are efficient under lower-temperature conditions, which would better align with green and ene...
