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Open access Sep 2026

One‐Pot RPA‐CRISPR/Cas12a Assay With Visual Readout for the Ultra‐Specific Detection of Monkeypox Virus Clade I

ABSTRACT In 2024, Monkeypox virus (MPXV) clade I has triggered outbreaks in several countries world‐wide. MPXV clade I demonstrates enhanced virulence and transmissibility, with a case fatality rate reaching 10%. In response, we have developed a one‐pot detection assay specifically targeting MPXV clade I, combining rec...

Bo-Yi Li, Lu-Yao Liu, Kai-Kai Jin et al. · 0 citations
Review Aug 2026

CRISPR/Cas trans-cleavage activity in pathogen detection: research progress and innovations.

The integrated CRISPR/Cas detection workflow from sample collection and processing through final result output is assessed, and the intrinsic characteristics of Cas effector proteins with respect to target enrichment, reporter molecules, readout formats, sample background, and validation design are analyzed.

Rong Gao, Hong-Li Jin, Tian-Yi Zhang et al. · 0 citations

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