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Pohl Milón

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Open access Sep 2026

Buffer tolerance landscape of LbCas12a trans-cleavage efficiency

Numerous CRISPR-Cas systems have been developed for molecular detection of genetic elements exploiting the trans-cleavage activity of LbCas12a combined with short fluorescent probes. Alongside, a large variability of buffer conditions has been reported. However, how solution chemistry balances enzymatic turnover, signa...

Roberto Alcántara, Roberto Spurio, Pohl Milón · 0 citations

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