A Step-by-Step Protocol for Quantifying Molecular Forces in Living Cells Using the Vinculin TSMod FRET Tension Sensor.
Genetically encoded FRET-based tension sensors provide a powerful approach for visualizing and quantifying molecular forces within living cells. These probes incorporate an extensible, calibrated elastic linker between a donor-acceptor fluorophore pair, enabling mechanical load changes to be reported as variations in F...