ABSTRACT Enterococcus faecalis is a gram-positive commensal bacterium of the human gut microbiome and an opportunistic pathogen responsible for many hospital-acquired infections. Despite the clinical importance of E. faecalis, how gene and protein expression are coordinated with growth remains poorly defined. Here, we...
M. M. Mitchener, Caleb M. Anderson, Mark Veleba et al.· mBio· 0 citations
In eubacteria, decoding of isoleucine codon AUA requires a specialized tRNA (tRNAIle2) modified with lysidine (k2C) at the anticodon wobble position (C34), which switches decoding specificity from methionine (AUG) to isoleucine (AUA). Recently, aminovaleramide cytidine (ava2C) was discovered at the same tRNA position i...
Jing-Jing Sun, Jun-Zhou Wu, Yi-Feng Yuan et al.· Nature Chemical Biology· 0 citations
ABSTRACT Enterococcus faecalis is a gram-positive commensal bacterium of the human gut microbiome and an opportunistic pathogen responsible for many hospital-acquired infections. Despite the clinical importance of E. faecalis, how gene and protein expression are coordinated with growth remains poorly defined. Here, we...
M. M. Mitchener, Caleb M. Anderson, Mark Veleba et al.· mBio· 0 citations
Transfer RNA (tRNA) modifications regulate codon-biased translation and enable rapid translational adaptation to environmental stresses in diverse organisms. While these mechanisms have been extensively characterized in Saccharomyces cerevisiae, little is known about the epitranscriptomic landscape of nonconventional...
Alexander J. B. Reading, Chi-Kong Chan, R. Raman et al.· Chemical Research in Toxicol...· 0 citations
Mass spectrometry (MS)-based nucleic acid analysis provides direct chemical evidence for oligonucleotide sequence, composition, and modifications. However, oligonucleotide LC-MS analysis commonly relies on ion-pairing reversed-phase liquid chromatography (IP-RPLC). Although IP-RPLC provides strong retention and high-re...
Jun-Zhou Wu, Rana Togay, Jing-Jing Sun et al.· bioRxiv· 0 citations
It is demonstrated that maximizing recombinant protein production requires optimization of both yield and structural quality and establish complementary strategies for improving secreted protein expression in Pichia.
