2026
An Efficient Colony PCR Method for Construct Verification in the Basidiomycete Ustilago maydis.
A straightforward and reliable protocol for DNA isolation and storage in the screening of U. maydis mutants, enabling the reproducible amplification of 0.5-5 kb fragments directly from fresh colonies grown on agar plates.
Pascal Künzel, Andreas Müsgens, Lars M. Blank et al.
· Methods in molecular biology · 0 citations