Author

P. Westh

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Review Open access Aug 2026

Mapping bacterial cutinase sequence space by high-throughput screening reveals that PET hydrolysis is a rare property

Poly(ethylene terephthalate) (PET) is one of the most widely produced plastics, and enzymatic depolymerization offers a promising route to closed-loop recycling under mild conditions. However, most known bacterial PET hydrolases belong to a conserved canonical-fold cutinase family, leaving much of α/β-hydrolase diversi...

Robin Dorau, M. B. Keller, E. M. Thiesen et al. · bioRxiv · 0 citations

Open access Sep 2026

EnZight: A Structure-Guided Algorithm to Identify and Prioritize Substitution Hotspots for Enzyme Engineering

Homologous protein structures contain valuable information about tolerated sequence variation. However, translating this information into practical enzyme design strategies remains challenging. Here we present EnZight, a user-friendly web server that integrates homologous structural alignment with intuitive visualizati...

R. R. Østergaard, Mikkel Lyskjær Jensen, Suzana Siebenhaar et al. · bioRxiv · 0 citations