P. V. van Veelen

Chemogenetic ubiquitin E1 inhibitor TAK-243 CRISPR screen uncovers HAPSTR1/HUWE1 axis in chromatin proteostasis and cell cycle control

In this study, we sought to comprehensively map ubiquitin signaling networks at the crossroads of cellular proliferation and proteostasis, both crucial in cancer. Genome-wide CRISPR knockout screening in the presence of the ubiquitin E1 inhibitor TAK-243 identified 239 genes whose ablation sensitizes cells to ubiquitin...

Selectivity and dynamics of VAP family tether exchange across membrane contact sites

Immunoglobulin constant domains as targets for T-cell receptor-based treatment of multiple myeloma.

Despite major progress with chimeric antigen receptor (CAR) T-cell therapy, multiple myeloma (MM) remains largely incurable, and most patient relapse. To broaden therapeutic options and overcome antigen escape, we explored T-cell receptor (TCR)-based targeting of intracellular antigens derived from immunoglobulin (Ig)...

CRISPR activation screen uncovers MARCKSL1 as a gauge for extracellular vesicle secretion.

How cells balance these competing routes using a genome-wide CRISPR activation screen for factors that alter surface expression of CD63-an EV-associated tetraspanin that shuttles between the PM and endosomes is investigated.

Identification of cellular intermediates unveils unique enzymes for flagellar glycan biosynthesis in Clostridioides difficile.

This study chemically synthesized two hitherto undescribed biosynthetic intermediates that were predicted based on bioinformatic analyses, CDP-threonine and CDP-N-methylthreonine, and showed that they are involved in the Type A PGM biosynthesis, as evidenced by mass spectrometric analyses of extracts of a set of C. dif...