Microbial halogenated natural products (hNPs) hold ecological, agricultural, and biomedical relevance. The hNP-producing potential of an organism can be assessed by the precise prediction of halogenating enzymes, yet detailed annotations of halogenases are often missing from genomic and metagenomic data. We created a m...
Judit Szenei, Ashleigh Burke, Anne Liong et al.· ACS Omega· 0 citations
Acetate is a promising carbon source for microbial biotechnology as it can be produced sustainably from lignocellulosic biomass or C1 gases. Since acetate is directly activated to acetyl-CoA, it is especially suitable for producing acetyl-CoA-derived products, showcased here with the production of 3-(3-hydroxyalkanoylo...
M. Filbig, Luisa Wachtendonk, Luca A. I. Hampe et al.· bioRxiv· 0 citations
Acetate is an attractive renewable two-carbon substrate for microbial biotechnology, but its toxicity limits growth and carbon-use efficiency at process-relevant concentrations. Here, we used adaptive laboratory evolution to improve acetate tolerance in a genome-reduced strain of Pseudomonas putida and combined whole-g...
N. Gurdo, A. Srinivasan, Tommaso Tagliani et al.· bioRxiv· 0 citations
MORTISE is introduced, a compact Cas6f-based RNA system to repress target bacterial genes without relying on host RNA factors, enables multiplex control across bacteria, and supports pathway balancing when genetic knockouts are unsuitable.
