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P. Neubauer

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Open access Aug 2026

Automated high-throughput screening combined with model-assisted analysis enables quantitative identification of process drivers and trade-offs during extracellular Fab production in Escherichia coli

Production of Fab antibody fragments with Escherichia coli strains engineered for extracellular product release can reduce downstream processing requirements but often compromises cellular integrity, resulting in increased cell lysis. To investigate the balance between product formation, product release, and cellular r...

F. Schröder-Kleeberg, L. Kaspersetz, Sanchir Anar Neff et al. · 0 citations
Open access Jul 2026

Genomically integrated orthogonal translation system in Escherichia coli enables production of functional modified [NiFe]-hydrogenases

The present work lays the groundwork for the bio-orthogonal engineering of hydrogenases and related hybrid biocatalysts by employing CRISPR/Cas9-mediated genome editing to integrate a psychrophilic pyrrolysyl-tRNA synthetase/tRNA pair into the Escherichia coli BL21 genome.

Qin Fan, Stevanie Stevanie, S. Frielingsdorf et al. · 0 citations

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