MILK is presented, a scalable computational framework that organizes high-dimensional single-cell populations into unified tree representations that establish the hierarchical organization of biological data as a scalable and unifying representation of cellular identity, enabling integrative analysis of single-cell gen...
Brett Kiyota, Chaehyeon Lee, Hao-Yang Yao et al.· bioRxiv· 0 citations
Autophagy is initiated at endoplasmic reticulum (ER)-associated omegasomes, yet how the ER contributes to mitochondrial cargo degradation during early autophagosome formation remains unclear. Gp78 is an ER-resident RING E3 ubiquitin ligase involved in ER-associated degradation (ERAD) that also promotes Parkin-independe...
Milene Ortiz-Silva, Bharat Joshi, Jerry Zheng et al.· bioRxiv· 0 citations
Cells in multicellular eukaryotic systems are diverse biological units, with characteristics and functions determined by their molecular profiles. CRISPR–Cas9 genome editing has been widely used across biology to modulate gene expression and study gene function. However, there is currently no versatile and scalable met...
Arman Adel, Yuta Shuto, Shunsuke Kawasaki et al.· bioRxiv· 0 citations
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