On-site and visual detection of genotype VII Newcastle disease virus based on RT-RPA and CRISPR/Cas13a
This rapid and highly sensitive diagnostic assay was established for genotype VII NDV by integrating reverse transcription recombinase polymerase amplification with the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas13a system, in combination with a lateral flow dipstick (LFD) for visual signal r...